膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is a mouse monoclonal antibody recombinantly expressed from CHO cells immunized with IgG Fc.
亚型（Isotype）
Mouse IgG1 | Kappa
特异性（Specificity）
This antibody specifically reacts with Human IgG Fc.
应用（Application）
ELISA
纯度（Purity）
>95% as determined by SDS-PAGE.
>98% as determined by SEC-MALS.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20 to -70°C for 12 months in lyophilized state from date of receipt;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product can cross in Elisa with
Human ACE2, Fc Tag (Cat. No. AC2-H5257).
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (Cat. No. PD-M401a).
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (Cat. No. SPD-M400a).
Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat. No. SPD-M402a).
This product No cross-reactivity in ELISA with
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162).
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Cynomolgus IgG1 (AM122) (Cat. No. SPD-M201).
Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2).
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgA1 (AM130) (Cat. No. S1N-M164).
Anti-SARS-CoV-2 Omicron Antibody-3A7C12, Rabbit IgG (Cat. No. SPD-C73).
电泳（SDS-PAGE）
Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-S307) is more than 98% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified) (Cat. No. SPD-M402a) at 2μg/mL (100μL/well) can bind Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (6F11C8)(Cat. No. IGG-S307) with a linear range of 0.05-3.13 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (MALS verified) (Cat. No. SPD-M400a), Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (MALS verified) (Cat. No. SPD-M401a), Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified) (Cat. No. SPD-M402a) and Human ACE2 / ACEH Protein, Fc Tag (MALS verified) (Cat. No. AC2-H5257) can bind Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (6F11C8)(Cat. No. IGG-S307). The antibody does not bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgA1 (AM130) (MALS verified) (Cat. No. S1N-M164) and Human CD19 (20-291) Protein, His Tag DMF Filed (Cat. No. CD9-H52H2) (Routinely tested).
Protocol
背景（Background）
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).
