登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Nectin-4 >NE4-C5253

Cynomolgus Nectin-4 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

NECTIN4,LNIR, PRR4, PVRL4

表达区间及表达系统(Source)

Cynomolgus Nectin-4 Protein, Fc Tag (NE4-C5253) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # XP_005541277.1).

Predicted N-terminus: Gly 32

Request for sequence

蛋白结构(Molecular Characterization)

Nectin-4 Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 60.5 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Nectin-4 SDS-PAGE

Cynomolgus Nectin-4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Nectin-4 SEC-MALS

The purity of Cynomolgus Nectin-4 Protein, Fc Tag (Cat. No. NE4-C5253) is more than 85% and the molecular weight of this protein is around 110-140 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Nectin-4 ELISA

Immobilized Cynomolgus Nectin-4 Protein, Fc Tag (Cat. No. NE4-C5253) at 5 μg/mL (100 μL/well) can bind Anti-Nectin-4 Antibody, Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

背景(Background)

Nectin-4(Nectin cell adhesion molecule 4) is a Nectin and nectin-like family of cell adhesion molecules. It is found in high levels in normal embryonic and fetal tissues, but decreases after adulthood and is limited in distribution in healthy tissues. Nectin-4 is overexpressed in a variety of tumor cells and promotes tumor cell growth and proliferation. Solid tumors with high expression of Nectin-4 include bladder cancer, pancreatic cancer, triple negative breast cancer, non-small cell lung cancer, stomach cancer, esophageal cancer, ovarian cancer and many other cancers. Studies have found that Nectin-4 promotes tumor cell proliferation, differentiation, migration and invasion by activating the PI3K/Akt pathway, and is believed to play an important role in the occurrence and metastasis of cancer. It is based on these research backgrounds that drug research targeting this target has emerged.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Nectin-4靶点信息
英文全称:Nectin-4
中文全称:细胞黏附分子nectin-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:10详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细
相关产品
View All Nectin-4

消息提示

请输入您的联系方式,再点击提交!

确定