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Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA)

热销产品推荐:The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

ItemsDetails
Detection MethodChemiluminescence
Product TypeMagnetic Beads (Monoclonal anti-human IgM antibody)
Quantity Size10mg / 100mg
Physical Appearancelyophilized powder mixture
Particle size2.8 µm
Beads SurfaceHydrophilic
Amount of Coupled ProteinAbout 133 pmol (20 μg) Anti- Human IgM Antibody / mg Beads
Binding Capacity3-8 μg Human IgM / mg beads
Emission WavelengthMeasured relative light units (RLU) at 430 nm

背景(Background)

The Anti-Human IgM-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-human IgM antibody. The beads can be used to capture the Human IgM in Chemiluminescence procedures. It has no-corss-reactivity with Human IgG, IgA or IgE. The Anti-Human IgM-coupled Magnetic Beads is easy to capture the human IgM, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

应用说明(Application)

The Anti-Human IgM-coupled Magnetic Beads is used to capture the human IgM, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.

*The Isotype control (Cat. No. SMB-B01) is sold separately.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

制剂(Formulation)

Please contact us for detailed information.

Contact us for customized product form or formulation.

 

活性(Bioactivity)-MPCLIA

Anti-Human IgM MPCLIA

Immobilized 0.04 μg /Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridine ester (Cat. No. STN-NA114, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162) at increasing concentration coupled to Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010, 10 μg /Test). Detection was performed with sensitivity of 0.244 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

Protocol

Anti-Human IgM MPCLIA

The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable at 37°C for 10 days.

Anti-Human IgM MPCLIA

The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable after freezing and thawing 3 times.

Anti-Human IgM MPCLIA

The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable at 37°C for 10 days.

 

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