膜杰作
Star Staining
描述（Description）
The CHO/Human LIGHT Stable Cell Line was engineered to express full length human LIGHT (Gene ID: 8740). Surface expression of human LIGHT was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based LIGHT binding assay
• Useful as LIGHT-expressing target cells in reporter gene assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
F-12K + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human LIGHT on CHO/Human LIGHT Stable Cell Line by FACS.
CHO/Human LIGHT Stable Cell Line or negative control cell were stained with PE-labeled anti-Human LIGHT
Protocol
Signaling Bioassay
Response to human HVEM (FOLD).
This cell was incubated with Human HVEM (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF105). The human LIGHT overexpressing on CHO cells can activate HVEM signaling with the max induction fold 227.59.
Protocol
Passage Stability
Passage stability analysis of receptor expression by FACS.
Flow cytometry surface staining of human LIGHT on CHO/Human LIGHT Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 4-24
Protocol
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背景（Background）
Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
