膜杰作
Star Staining
分子别名（Synonym）
CD5,LEU1
表达区间及表达系统（Source）
Rhesus macaque CD5 Protein, His Tag (CD5-R52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Thr 382 (Accession # F6V5Q9).
Predicted N-terminus: Arg 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 41.3 kDa. The protein migrates as 47-52 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rhesus macaque CD5 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rhesus macaque CD5 Protein, His Tag (Cat. No. CD5-R52H3) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rhesus macaque CD5 Protein, His Tag (Cat. No. CD5-R52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD5 Antibody, Mouse IgG1 (2C8A7) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
背景（Background）
T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1,which is phosphorylated on tyrosine residues by LYN,so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development,selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.
