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ClinMax™ Human Soluble Delta Like Protein 4 (DLL4) ELISA Kit, PRO

For research use only.

产品概述(Product Details)

ClinMax™ Human Soluble Delta Like Protein 4 (DLL4) ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Delta Like Protein 4 (DLL4) that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human Delta Like Protein 4 (DLL4) in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

DLL4 Workflow

关键信息(Key Features)

AnalyteSoluble Delta Like Protein 4 (DLL4)
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity5.0 pg/mL
Range15.625 pg/mL-1000 pg/ml
Assay Time1.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

DLL4 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of DLL4 is less than 5.0 pg/mL.

 

验证(Validation)

批内差异(Intra-Assay Statistics)

Ten replicates of each of 4 samples containing different DLL4 concentrations were tested in one assay. Acceptable criteria: CV<10%.

DLL4 INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

Five samples containing different concentrations of DLL4 were tested in independent assays. Acceptable criteria: CV<15%.

DLL4 INTER-ASSAY STATISTICS

回收率(Recovery)

Recombinant DLL4 was spiked into 3 human serum samples, and then analyzed. The average recovery of DLL4 for serum samples is 89.2%.

DLL4 RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA038-C01Pre-coated Anti-DLL4 Antibody Microplate1 plate
CEA038-C02Human DLL4 Standard100 μg ×2
CEA038-C03Biotin-Anti-DLL4 Antibody Con. Solution400 μL
CEA038-C04Biotin-Antibody Dilution Buffer8 mL
CEA038-C05Streptavidin-HRP Con. Solution500 μL
CEA038-C06Streptavidin-HRP Dilution Buffer15 mL
CEA038-C0720× Washing Buffer50 mL
CEA038-C08Sample Dilution Buffer15 mL ×2
CEA038-C09Substrate Solution12 mL
CEA038-C10Stop Solution6 mL

 

前沿进展

 
 
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010-53681107(北京)
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DLL4靶点信息
英文全称:Delta-like protein 4
中文全称:δ样蛋白-4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床三期
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