膜杰作
Star Staining
分子别名（Synonym）
IGFBP4,IBP-4
表达区间及表达系统（Source）
Biotinylated Human IGFBP-4 Protein, His,Avitag (IG4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1).
Predicted N-terminus: Asp 22
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 29.6 kDa. The protein migrates as 35-40 kDa and 41-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IGFBP-4 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IGFBP-4 Protein, His,Avitag (Cat. No. IG4-H82E3) is more than 90% and the molecular weight of this protein is around 28-40 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IGF-II, Fc Tag (Cat. No. IG2-H4260) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-4 Protein, His,Avitag (Cat. No. IG4-H82E3) with a linear range of 2-40 ng/mL (QC tested).
Protocol
Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-4 Protein, His,Avitag (Cat. No. IG4-H82E3) with a linear range of 2-40 ng/mL (Routinely tested).
Protocol
背景（Background）
Insulin-like growth factor-binding protein 4 (IGFBP-4) is also known as IGF-binding protein 4, which contains an IGFBP domain and a thyroglobulin type-I domain. IGFBP-4 binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. IGFBP-4 is secreted by all colon cancer cells.
