膜杰作
Star Staining
分子别名（Synonym）
HLA-A*0201 & B2M & PAP (ALDVYNGLL)
表达区间及表达系统（Source）
PE-Labeled Human HLA-A*02:01&B2M&PAP (ALDVYNGLL) Tetramer Protein (HLP-HP2Hb) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ALDVYNGLL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ALDVYNGLL).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
PE-Labeled Human HLA-A*02:01&B2M&PAP (ALDVYNGLL) Tetramer Protein is assembled by biotinylated monomer and PE-labeled streptavidin.
Biotinylated Human HLA-A*02:01&B2M&PAP (ALDVYNGLL) Complex Protein is produced by co-expression of HLA and B2M loaded with PAP peptide. Biotinylated Human HLA-A*02:01&B2M&PAP (ALDVYNGLL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized PE-Labeled Human HLA-A*02:01&B2M&PAP (ALDVYNGLL) Tetramer Protein (Cat. No. HLP-HP2Hb) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized PE-Labeled Human HLA-A*02:01&B2M&PAP (ALDVYNGLL) Tetramer Protein (Cat. No. HLP-HP2Hb) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
背景（Background）
Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor
