膜杰作
Star Staining
产品参数（Product Specifications）
| Assay Type | Sandwich-ELISA |
| Analyte | Pyrophosphatase |
| Format | 96T(8×12 strips) |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | < 9.38 pg/mL |
| Standard Curve Range | 9.375 pg/mL-300 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of Pyrophosphatase in Cell Culture Supernatants. |
| Sample volume | 100 μL |
产品概述（Product Overview）
resDetect™ Pyrophosphatase ELISA Kit is based on the ELISA sandwich method and is used to detect and quantitatively determine Pyrophosphatase residues in mRNA drug products or semi-manufactures. It contains Pyrophosphatase Standard (ACROBiosystems, cat# PYE-T5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling Pyrophosphatase residues during the production of mRNA preparations. It can also be used as a universal detection tool for the quantitative determination of Pyrophosphatase.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
2-8℃
组分（Materials Provided）
| ID | Components | Size |
| RES005-C01 | Pre-coated Anti-Pyrophosphatase Antibody Microplate | 1 plate(8×12 strips) |
| RES005-C02 | Pyrophosphatase Standard | 100 μL |
| RES005-C03 | Biotin-Anti-Pyrophosphatase Antibody | 15 μg |
| RES005-C04 | Streptavidin-HRP | 50 μL |
| RES005-C05 | 20xWashing Buffer | 50 mL |
| RES005-C06 | 2xDilution Buffer | 50 mL |
| RES005-C07 | Substrate Solution | 12 mL |
| RES005-C08 | Stop Solution | 7 mL |
质量管理控制体系（QMS）
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
验证（Validation）
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<15%.
回收率（Recovery）
Three Pyrophosphatase with different concentrations were tested to calculate the recovery rate.
产品推荐（Recommended Products）
背景（Background）：Pyrophosphatase
Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate, it can hydrolyze inorganic pyrophosphate generated with the reaction to avoid its inhibition of the reaction system. Pyrophosphatase is usually used to increase RNA yield in reverse transcription reactions. As a key raw material for in vitro transcription (IVT) of RNA, Pyrophosphatase needs to detect the residues as a protein component. This ELISA kit can be used to detect the residue of Pyrophosphatase in mRNA stock solution.
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