分子别名(Synonym)
Complement C3,CPAMD1
表达区间及表达系统(Source)
Human Complement component 3 Protein, Fc Tag (CO3-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1).
Predicted N-terminus: Ser 23
Request for sequence
蛋白结构(Molecular Characterization)
![Complement C3 Structure](/images/structure_Images/CO3-H5253-STC2.jpg)
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 211.4 kDa. The protein migrates as 75-80 kDa,160-180 kDa and >180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
![Complement C3 SDS-PAGE](images/sds/CO3-H5253.jpg)
Human Complement component 3 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
![Complement C3 SEC-MALS](images/mals/CO3-H5253-M1.jpg)
The purity of Human Complement component 3 Protein, Fc Tag (Cat. No. CO3-H5253) is more than 85% and the molecular weight of this protein is around 390-440 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
![Complement C3 ELISA](images/elisa/CO3-H5253-E1.jpg)
Immobilized Human Complement component 3 Protein, Fc Tag (Cat. No. CO3-H5253) at 1 μg/mL (100 μL/well) can bind Anti-C3 antibody with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
背景(Background)
C3 is the major complement component serum. It is mainly synthesized by macrophages and liver. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. It is cleaved into two fragments, C3a and C3b. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.