描述(Description)
The HEK293/Human TL1A Stable Cell Line was engineered to express the receptor full length human TL1A (Gene ID:9966). Surface expression of human TL1A was confirmed by flow cytometry.
应用说明(Application)
• Useful for cell-based TL1A binding assay
生长特性(Growth Properties)
Adherent
筛选标记(Selection Marker)
Puromycin (2 μg/mL)
培养基(Culture Medium)
DMEM + 10% FBS
冻存液(Freeze Medium)
Serum-free cell cryopreservation medium
装量(Quantity)
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储(Storage)
Frozen in liquid nitrogen.
支原体检测(Mycoplasma Testing)
Negative
无菌检测(Sterility Testing)
Negative
使用说明(Instructions for Use)
See data sheet for detailed culturing and assay protocol.
Receptor Assay
![TL1A FACS](images/facs/CHEK-ATP142-F1.jpg)
Expression analysis of human TL1A on HEK293/Human TL1A Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human TL1A Stable Cell Line or negative control cell using anti-human TL1A Antibody followed by staining with PE anti-human IgG Fc Antibody.
Protocol
Passage Stability
![TL1A PASSAGE](images/cell/CHEK-ATP142-CD1.jpg)
Passage stability analysis of receptors expression by FACS.
Flow cytometry surface staining of human TL1A on HEK293/Human TL1A Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 5-24.
Protocol
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背景(Background)
TNF-like cytokine 1A (TL1A) and its receptors, death receptor 3 (DR3) and decoy receptor 3 (DcR3) are members of the TNF and TNF receptor superfamilies of proteins, respectively. Binding of APC-derived TL1A to lymphocytic DR3 provides co-stimulatory signals for activated lymphocytes. DR3 signaling affects not only the proliferative activity of and cytokine production by effector lymphocytes, but also critically influences the development and suppressive function of regulatory T-cells. Whereas, DcR3 restricts the function of the TL1A/DR3 complex: attenuating T-cell activation and downregulating the secretion of pro-inflammatory cytokines. Together with DR3 and DcR3, TL1A constitutes a cytokine system that actively interferes with the regulation of immune responses.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.