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 >  Protein>GAS6 >GA6-H5246

Human GAS6 (49-678) Protein, His Tag

分子别名(Synonym)

GAS6,GAS-6,AXLLG

表达区间及表达系统(Source)

Human GAS6 Protein, His Tag (GA6-H5246) is expressed from human 293 cells (HEK293). It contains AA Ala 49 - Ala 678 (Accession # Q14393-2).

Request for sequence

蛋白结构(Molecular Characterization)

GAS6 Structure

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 71.8 kDa. The protein migrates as 80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

GAS6 SDS-PAGE

Human GAS6 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

GAS6 ELISA

Immobilized Human GAS6 Protein, His Tag (Cat. No. GA6-H5246) at 5 μg/mL (100 μL/well) can bind Human Axl, Fc Tag (Cat. No. AXL-H5253) with a linear range of 0.06-1 ng/mL (QC tested).

Protocol

GAS6 ELISA

Immobilized Human GAS6 Protein, His Tag (Cat. No. GA6-H5246) at 10 μg/mL (100 μL/well) can bind Human TYRO3, Fc Tag (Cat. No. TY3-H5251) with a linear range of 2-20 ng/mL (Routinely tested).

Protocol

GAS6 ELISA

Immobilized Human GAS6 Protein, His Tag (Cat. No. GA6-H5246) at 10 μg/mL (100 μL/well) can bind Human MERTK, Fc Tag (Cat. No. MEK-H5253) with a linear range of 0.04-1.3 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

GAS6 SPR

Human Axl, Fc Tag (Cat. No. AXL-H5253) captured on Protein A Chip can bind Human GAS6 Protein, His Tag (Cat. No. GA6-H5246) with an affinity constant of 38.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

GAS6 (Growth arrest-specific protein 6) is also known as AXL receptor tyrosine kinase ligand, AXLLG. Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

 

前沿进展

 
 
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GAS6靶点信息
英文全称:Growth arrest-specific protein 6
中文全称:生长停滞特异性蛋白6
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床三期
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