膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The ISRE (Luc) HEK293 Reporter Cell was engineered to express IFN-stimulated response elements (ISRE) driving luciferase expressing systems. When stimulated with human Type I interferons (IFNs), receptor-mediated signaling can drive IFN-stimulated gene factor 3 (ISGF3)-mediated luminescence.
应用说明（Application）
• Screen for activators or inhibitors of Type I interferons.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Signaling Bioassay
Response to human IFN-alpha 2b protein (RLU).
The ISRE (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IFN-alpha 2b protein (Cat. No. IFB-H5253). The EC50 was approximately 0.021 μg/mL.
Protocol
Response to human IFN-alpha 2b protein (FOLD).
The ISRE (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IFN-alpha 2b protein (Cat. No. IFB-H5253). The max induction fold was approximately 107.
Protocol
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背景（Background）
ISRE is a marker of interferon stimulated genes (ISGs), which mainly include enzymes that can inhibit virus replication. Type I (such as IFN α and IFN β） and type III interferon (such as IFN-λ) can activate STAT1 and STAT2, and the activated STAT1, STAT2, and IRF9 form a protein complex called ISGF3. The ISGF3 protein complex binds to the promoter containing the ISRE element (interferon-stimulated response element) and activates the expression of related genes.
Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
