膜杰作
Star Staining
分子别名（Synonym）
FGF-5,Fibroblast growth factor 5,HBGF-5
表达区间及表达系统（Source）
Human FGF-5 Protein, His Tag (FG5-H51H3) is expressed from E. coli cells. It contains AA Glu 23 - Gly 268 (Accession # P12034-1).
Predicted N-terminus: Glu 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human FGF-5 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-SPR
Human FGF-5 Protein, His Tag (Cat. No. FG5-H51H3) captured on CM5 chip via anti-His antibody can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 63.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
Fibroblast growth factor-5 (FGF5) is a member of the fibroblast growth factor (FGF) family and acts as a crucial regulator of hair growth and an oncogenic factor in several human cancers. Generally, Fibroblast growth factor 5 (FGF5) is widely expressed in embryonic but scarcely in adult tissues. Overexpression of FGF5 has been associated with prostate cancer, pancreatic cancer, breast cancer, renal cell carcinoma, etc. it was confirmed that FGF5 contributes to the malignant progression of human astrocytic brain tumors by both autocrine and paracrine effects.
