膜杰作
Star Staining
分子别名（Synonym）
EGFR,ERBB,ERBB1,HER1,PIG61,mENA
表达区间及表达系统（Source）
Biotinylated Mouse EGF R Protein, Fc,Avitag (EGR-M82F3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1).
Predicted N-terminus: Leu 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 97.4 kDa. The protein migrates as 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Mouse EGF R Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Mouse EGF R Protein, Fc,Avitag (Cat. No. EGR-M82F3) is more than 85% and the molecular weight of this protein is around 240-280 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse EGF R Protein, Fc,Avitag (Cat. No. EGR-M82F3) with a linear range of 0.5-4 ng/mL (QC tested).
Protocol
Immobilized cetuximab at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse EGF R Protein, Fc,Avitag (Cat. No. EGR-M82F3) with a linear range of 0.016-1 μg/mL (Routinely tested).
Protocol
Immobilized Monoclonal Anti-Human EGF R Antibody, Human IgG1 (Cat. No. EGR-M1201) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse EGF R Protein, Fc,Avitag (Cat. No. EGR-M82F3) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
背景（Background）
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
