膜杰作
Star Staining
分子别名（Synonym）
CD3 epsilon & CD3 gamma,CD3E & CD3G
表达区间及表达系统（Source）
Marmoset CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-M5253) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 51 - Ser 139 (CD3G) (Accession # Q3LRP4-1 (CD3E) & A0A2R8MU93-1 (CD3G)).
Predicted N-terminus: Gln 22 (CD3E) & Gln 51 (CD3E)
Request for sequence
蛋白结构（Molecular Characterization）
Marmoset CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 37.4 kDa (CD3E) & 36.4 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 41-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Marmoset CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Marmoset CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-M5253) is more than 90% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Marmoset CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-M5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
背景（Background）
T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
