Anti-SARS-CoV-2 (XBB.1.5) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)

背景（Background）

XBB.1.5 was first identified in the United States in New York in October 2022. Both XBB and the Kraken version (XBB.1.5) are recombinant (or hybrid) virus subvariants, meaning they are made up of two strains—in this case, two offshoots of the Omicron BA.2 sublineage.

To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure neutralizing antibodies against the mutants is in urgent need.

应用说明（Application）

The kit is developed for titer measurement of Anti-SARS-CoV-2 (XBB.1.5) neutralizing antibody (Spike RBD) in the sample.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody through a competitive ELISA. The microplate in the kit has been pre-coated with Human ACE2 protein. First serum samples, Positive control, Negative Control are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. After incubation, the wells are washed and substrate is added to the wells. The reaction is terminated by the addition of stop solution and the intensity of color is measured at 450 nm. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Dilute the HRP-SARS-CoV-2 RBD,samples and the Controls with Dilution Buffer.

c) Add samples,the Controls and the HRP-SARS-CoV-2 RBD to the plate respectively.

d) Wash the plate and add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.