登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>DR3 >DR3-H5253

Human DR3 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

TNFRSF25,DR3,APO3,DDR3,TNFRSF12,WSL,WSL1

表达区间及表达系统(Source)

Human DR3 Protein, Fc Tag (DR3-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).

Request for sequence

蛋白结构(Molecular Characterization)

DR3 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 45.5 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

DR3 SDS-PAGE

Human DR3 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

DR3 SEC-MALS

The purity of Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

DR3 ELISA

Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 1-78 ng/mL (QC tested).

Protocol

DR3 ELISA

Immobilized Human DR3, Fc Tag (Cat. No. DR3-H5253) at 5 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-TL1A Neutralizing Antibody, Human lgG1 and 1 μg/mL (50μL/well) Biotinylated Human TL1A, His Avitag (Cat.No.TLA-H52Q1) with a half maximal inhibitory concentration (lC50) of 1.95 μg/mL (Routinely tested).

Protocol

 

活性(Bioactivity)-SPR

DR3 SPR

Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 7.58 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细
相关产品
View All DR3

消息提示

请输入您的联系方式,再点击提交!

确定