膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | Capsid protein p24 |
| Format | 96T |
| Reactivity | HIV (HV1-HXB2) |
| Regulatory Status | RUO |
| Sensitivity | <7.81pg/mL |
| Standard Curve Range | 250-7.81pg/mL |
| Assay Time | 3hr 20 min |
| Suitable Sample Type | For the quantitative determination of HIV-1 p24 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 uL |
组分（Materials Provided）
| ID | Components | Size |
| RES050-C01 | Pre-coated Anti-HIV-1 p24 Antibody Microplate | 1 plate |
| RES050-C02 | HIV-1 p24 Standard | 20 μg |
| RES050-C03 | Biotin-Anti-HIV-1 p24 Antibody | 20 μg |
| RES050-C04 | Streptavidin-HRP | 50 μL |
| RES050-C05 | 10xWashing Buffer | 50 mL |
| RES050-C06 | 2xDilution Buffer | 50 mL |
| RES050-C07 | Substrate Solution | 12 mL |
| RES050-C08 | Stop Solution | 7 mL |
| RES050-C09 | 1xLysis Buffer | 12 mL |
背景（Background）
HIV-1 p24 ELISA Kit is based on ELISA sandwich method and designed to measure HIV-1 p24 levels in samples from CAR-T product preparation processing. It contains recombinant HIV-1 p24 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of HIV-1 p24 concentrations.
应用说明（Application）
This kit is developed for quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processing where it is used as a lentivirus vector.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
Unopened kit should be stored at 2°C -8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of HIV-1 p24. The kit consists of Pre-coated Anti-HIV-1 p24 Antibody Microplate and HIV-1 p24 Standard and Biotin-Anti-HIV-1 p24 Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the HIV-1 p24 as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-HIV-1 p24 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
背景（Background）：Capsid protein p24
Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. The gag gene of HIV-1 encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein, which is the main component of HIV virus and plays an important role in the process of virus reproduction. Usually, p24 protein can be detected in blood 2-3 weeks after hiv infection.Its measurement is commonly used as an indicator of HIV-1 infection and viral load. Therefore, it's helpful to develop the Human Immunodeficiency Virus type 1 (HIV-1) p24 ELISA Kit to quantitative detection the HIV-1 p24 antigen in cell culture supernates .
