膜杰作
Star Staining
分子别名（Synonym）
ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1
表达区间及表达系统（Source）
Biotinylated Cynomolgus Her2 Protein, Fc,Avitag (HE2-C82F3) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # XP_005584091.2).
Predicted N-terminus: Thr 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 97.5 kDa. The protein migrates as 110-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Cynomolgus Her2 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Trastuzumab Biosimilar at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus Her2 Protein, Fc,Avitag (Cat. No. HE2-C82F3) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Bispecific Antibody (Her2 × Her3) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus Her2 Protein, Fc,Avitag (Cat. No. HE2-C82F3) with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Cynomolgus Her2 Protein, Fc,Avitag (Cat. No. HE2-C82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (STN-N5116) can bind Trastuzumab Biosimilar with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
背景（Background）
Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .
