膜杰作
Star Staining
产品概述（Product Details）
ClinMax™ Human Soluble NT5E/CD73 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human NT5E/CD73 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human NT5E/CD73 in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
| Analyte | Soluble NT5E/CD73 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 100 pg/mL |
| Range | 123 pg/mL-30000 pg/mL |
| Assay Time | 3hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of NT5E/CD73 is less than 100 pg/mL.
验证（Validation）
批内差异（Intra-Assay Statistics）
Ten replicates of each of 3 samples containing different NT5E/CD73 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
3 samples containing different concentrations of NT5E/CD73 were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
Recombinant NT5E/CD73 was spiked into 3 human serum samples, and then analyzed. The average recovery of NT5E/CD73 for serum samples is 93.0%.
组分（Materials Provided）
| ID | Components | Size |
| CEA048-C01 | Pre-coated Anti- Soluble NT5E/CD73 Antibody Microplate | 1 plate |
| CEA048-C02 | Human Soluble NT5E/CD73 Standard | 123μg ×2 |
| CEA048-C03 | Biotin-Anti-Soluble NT5E/CD73 Antibody Con. Solution | 200 μL |
| CEA048-C04 | Biotin-Antibody Dilution Buffer | 15 mL |
| CEA048-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA048-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA048-C07 | 20× Washing Buffer | 50 mL |
| CEA048-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA048-C09 | Substrate Solution | 12 mL |
| CEA048-C10 | Stop Solution | 6 mL |
