膜杰作
Star Staining
描述（Description）
The HEK293/Human PD-1 Stable Cell Line was engineered to express human PD-1 (AA sequence : Leu 25 - Leu 288, Protein Acc.# : NP_005009.2). Surface expression of Human PD-1 was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based PD-1 binding assay.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin B（300 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
10% DMSO + 90% FBS
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Fig. FACS analysis of PD-1 on HEK293/Human PD-1 Stable Cell Line.
FACS assay shows that Human PD-L1 Protein, Mouse IgG1 Fc Tag can bind to HEK293/Human PD-1 Stable Cell Line. HEK293/Human PD-1 Stable Cell Line was red line, negative control HEK293 cells was grey line(QC tested).
Protocol
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背景（Background）
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
