膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RIPO-HWM004-C01 | Basal medium M-M | 225ml |
| ID | Components | Size |
| RIPO-HWM004-1-C01 | Supplement M-M | 25ml |
产品描述（Product Details）
Cardiac organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the human heart. Human iPSC-Derived Cardiac Organoid maintenance Kit allows the maturation and the long-term maintenance of cardiac organoids.
产品特征（Product Specification）
The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect cardiac organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.
产品示意（Product Diagram）
Protocol Diagram of cardiac organoid differentiation.
验证数据（Validation Data）
Organoid Histology and State
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) show regular beating from day 7-13 of differentiation.
Marker Expression
The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) demonstrate the expression of cardiomyocyte marker cTnT (green).
Organoid Activity
The electrical activity of the cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) was recorded using silicon probes (Ca. No. P64-7), revealing electrophysiological activity that closely resembles that of the actual human heart.
