描述(Description)
HEK293/Human STEAP1 Stable Cell Line
应用说明(Application)
For cell-based binding assays
生长特性(Growth Properties)
Adherent
筛选标记(Selection Marker)
Puromycin (5 μg/mL)
培养基(Culture Medium)
DMEM medium + 10% FBS
冻存液(Freeze Medium)
10% DMSO + 90% FBS
装量(Quantity)
1 vial contains at least 5x10^6 cells in 1 ml of 10% DMSO in FBS.
存储(Storage)
Frozen in liquid nitrogen.
支原体检测(Mycoplasma Testing)
Negative
无菌检测(Sterility Testing)
Negative
使用说明(Instructions for Use)
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Fig. FACS analysis of STEAP1 on HEK293/Human STEAP1 Stable Cell Line.
FACS assay shows that Anti-STEAP1 antibody can bind to HEK293/Human STEAP1 Stable Cell Line. HEK293/Human STEAP1 Stable Cell Line was red line, Negative control HEK293 cells was grey line(QC tested).
Protocol
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背景(Background)
This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.