膜杰作
Star Staining
| ID | Components | Size |
| RAS184-C01 | Pre-coated SARS-CoV-2 Nucleocapsid Protein (BA.1) Microplate | 1 plate |
| RAS184-C02 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS184-C03 | SARS-CoV-2 Antibody Negative Control | 100 μL |
| RAS184-C04 | HRP-Conjugated Antibody | 50 μL |
| RAS184-C05 | 10 x Washing Buffer | 50 mL |
| RAS184-C06 | Dilution Buffer | 50 mL |
| RAS184-C07 | Substrate Solution | 12 mL |
| RAS184-C08 | Stop Solution | 7 mL |
背景（Background）
Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide.As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans.To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
应用说明（Application）
The kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (BA.1) antibody IgG (Nucleocapsid Protein) in mouse serum.
It is for research use only.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Nucleocapsid Protein.The Kit consists of Pre-coated with SARS-CoV-2 Nucleocapsid Protein Microplate,Positive Control,Negative Control and HRP-conjugated antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
