膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Antibody, Human IgG1 (8A1) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
8A1
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein is expressed from human 293 cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) and HA1.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.006-8 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Influenza A [A/Shanghai/2/2013(H7N9)] HA, Fc Tag (Cat. No. HA9-V5253).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).
Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).
电泳（SDS-PAGE）
Monoclonal Anti-Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Antibody, Human IgG1 (8A1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Antibody, Human IgG1 (8A1) (Cat. No. HA1-M694) is more than 90% and the molecular weight of this protein is around 140-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (Cat. No. HA1-V52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Antibody, Human IgG1 (8A1) (Cat. No. HA1-M694) with a linear range of 0.006-0.8 ng/mL (QC tested).
Protocol
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Antibody, Human IgG1 (8A1) (Cat. No. HA1-M694) with a linear range of 0.005-0.8 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Antibody, Human IgG1 (8A1) (Cat. No. HA1-M694) captured on Protein A Chip can bind Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] HA1 Protein, His Tag (Cat. No. HA1-V52H5) with an affinity constant of 2.10 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
- 774XXXXXXX
- I have been using the unconjugated FMC63 for a while and as soon as we knew about the conjugated FMC63 we wanted to use this as it will be saving us 1.5 hours in the staining procedure. We used this antibody and we were able to see a better seperation of positive and negative poulations. Its easy to use and 1:25 dilution works really well. I will recommend this 200%
>- 2022-2-26
- 156XXXXXXX8
- 该抗体目前主要用于细胞转染后阳性细胞转染效率检测，实验过程中抗体的高灵敏度及识别特异性，能够真实有效的反映出结果的准确性，且重复性，后期实验需要将会继续订购。
>- 2022-12-13
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背景（Background）
Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. HA molecules are proteolytically cleaved into HA1 and HA2 glycopolypeptides intra- or extracellularly, depending on the structure of the cleavage site.
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