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 >  Protein>IL-6 >IL6-H5243

Human IL-6 Protein, His Tag (MALS verified)

分子别名(Synonym)

IL6,Interleukin-6,BSF2,HSF,IFNB2

表达区间及表达系统(Source)

Human IL-6 Protein, His Tag (IL6-H5243) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # P05231-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

IL-6 Structure

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.7 kDa. The protein migrates as 25-27 kDa and 29-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-6 SDS-PAGE

Human IL-6 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

IL-6 SEC-MALS

The purity of Human IL-6 Protein, His Tag (Cat. No. IL6-H5243) is more than 85% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-6 ELISA

Immobilized Human IL-6 Protein, His Tag (Cat. No. IL6-H5243) at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, Fc Tag (Cat. No. ILR-H5259) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

 

背景(Background)

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

 

前沿进展

 
 
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IL-6靶点信息
英文全称:Interleukin-6
中文全称:白细胞介素-6
种类:Homo sapiens
上市药物数量:6详情
临床药物数量:18详情
最高研发阶段:批准上市
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