膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
5G3-1C6
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Chemical
免疫原（Immunogen）
PEG.
特异性（Specificity）
Specifically recognizes human PEG.
应用（Application）
| Application | Recommended Usage |
| ELISA | 5-625 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized PEG Coated Plates, Clear, 12×8-Well Strips, White Frame at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) (Cat. No. PEG-M687) with a linear range of 5-156 ng/mL (QC tested).
Protocol
Immobilized 5KD-PEG/20KD-PEG/40KD-PEG at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) (Cat. No. PEG-M687) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-MSD
MSD assay for the Detection of anti-IFN-PEG(Hide drug info) in Human Serum.
Immobilized Biotin Human IFN on MSD GoldTM 96-well Streptavidin Plate,can bind anti-IFN-PEG when detected by Sulfo Anti-PEG Antibody.Use the MESO QuickPlex SQ 120 MM instrument for analysis.
背景（Background）
Polyethylene glycol, referred to as PEG, is used as an inactive ingredient in the pharmaceutical industry as a solvent, plasticizer, surfactant, ointments, and suppository base, and tablet and capsule lubricant. PEG has low toxicity with systemic absorption less than 0.5%.
PEGylation occurs when PEGs are attached to various protein medications, allowing for greater solubility for certain drugs. Examples of PEGylated medications include PEG-interferon alpha (Pegintron) and PEG-filgrastim (Neulasta)
