膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RAS179-C01 | Pre-coated RABV Nucleoprotein Microplate | 1 plate |
| RAS179-C02 | RABV-N Antibody Positive Control | 50 μL |
| RAS179-C03 | RABV-N Antibody Negative Control | 50 μL |
| RAS179-C04 | HRP-Conjugated Antibody | 50 μL |
| RAS179-C05 | 10 x Washing Buffer | 50 mL |
| RAS179-C06 | Dilution Buffer | 50 mL |
| RAS179-C07 | Substrate Solution | 12 mL |
| RAS179-C08 | Stop Solution | 7 mL |
背景（Background）
Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.
应用说明（Application）
The kit is developed for titer measurement of Anti-RABV Nucleoprotein Antibody IgG in Mouse serum.
It is for research use only.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-RABV-N antibodies in Mouse serum by RABV Nucleoprotein. The Kit consists of Pre-coated RABV Nucleoprotein Microplate,Positive Control,Negative Control and HRP-conjugated antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
