膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RIPO-IWM005K-C01 | Intestinal organoid Basal Medium B | 14.5ml |
| RIPO-IWM005K-C02 | Intestinal organoid Basal Meidium C | 14.5ml |
| RIPO-IWM005K-C03 | Intestinal organoid Basal Medium D | 90ml |
| ID | Components | Size |
| RIPO-IWM005K-1-C01 | Intestinal organoid Medium A | 10ml |
| RIPO-IWM005K-1-C02 | Intestinal organoid Supplement B | 1.5ml |
| RIPO-IWM005K-1-C03 | Intestinal organoid Supplement C | 1.5ml |
| RIPO-IWM005K-1-C04 | Intestinal organoid Supplement D | 10ml |
产品描述（Product Details）
Human iPSC-Derived Intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) allows hESC or hiPSC to differentiate into intestine organoids. Intestine organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human intestine regions. This kit can produce 48 intestinal organoids in four steps. Organoids generated using Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) feature various types of cells, including intestine epithelium cells, mesenchyme cells, enterocytes, Paneth cells, goblet cells, etc. These intestine organoids show intestine crypt like structure, Villi and Microvilli like structure. These intestine organoids show normal intestinal function validated by the absorption of fatty acid and glucose.
产品特征（Product Specification）
The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.
产品示意（Product Diagram）
Protocol Diagram of intestine organoid differentiation.
验证数据（Validation Data）
Organoid Histology and State
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.
Orgnaoid Histology
Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 intestine organoids.
Marker Expression
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).
Organoid Activity
Intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.
