膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RIPO-RWM009K-C01 | Liver organoid Basal medium A | 9ml |
| RIPO-RWM009K-C02 | Liver organoid Basal medium B | 9ml |
| RIPO-RWM009K-C03 | Liver organoid Basal medium C | 13.5ml |
| RIPO-RWM009K-C04 | Liver organoid Basal medium D | 22.5ml |
| ID | Components | Size |
| RIPO-RWM009K-1-C01 | Liver organoid Supplement A | 1ml |
| RIPO-RWM009K-1-C02 | Liver organoid Supplement B | 1ml |
| RIPO-RWM009K-1-C03 | Liver organoid Supplement C-1 | 1.2ml |
| RIPO-RWM009K-1-C04 | Liver organoid Supplement C-2 | 0.3ml |
| RIPO-RWM009K-1-C05 | Liver organoid Supplement D-1 | 2ml |
| RIPO-RWM009K-1-C06 | Liver organoid Supplement D-2 | 0.5ml |
产品描述（Product Details）
Human iPSC-Derived Liver Organoid Differentiation Kit allows hESC or hiPSC to differentiate into liver organoids. Liver organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human liver. This kit can produce 48 liver organoids in four steps.
产品特征（Product Specification）
The basic medium of this kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.
产品示意（Product Diagram）
Protocol Diagram of liver organoid differentiation.
验证数据（Validation Data）
Orgnaoid Histology
H&E Staining of liver organoid.
Marker Expression
The liver organoids show expression of hepatocytes (HFN4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).
Pre-mature liver organoids show liver lobules-like structure.
Organoid Activity
Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.
Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.
