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Ready-to-use Human iPSC-Derived Liver Organoids

DS

产品描述(Product Details)

The Ready-to-use liver organoids are derived form human iPS cells and being cultured for more than 60 days. Organoids generated using Human iPSC-Derived liver organoid feature various types of cells, including hepatocytes, bile duct cells and vascular endothelial cells.

产品特征(Product Specification)

The live organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.

存储(Storage)

After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.

*请注意,为了支持完整的类器官培养和维持过程,本产品必须与Human iPSC-Derived Liver Organoid Expansion and Maintenance Medium(Cat. No RIPO-RWM010)一起使用。您可以点击此链接获取产品信息。
 

产品示意(Product Diagram)

Organoids PRODUCT DIAGRAM

Protocol Diagram of liver organoid differentiation.

验证数据(Validation Data)

Orgnaoid Histology

Organoids ORGNAOID HISTOLOGY

H&E Staining of liver organoid.

Marker Expression

Organoids MARKER EXPRESSION

The liver organoids show expression of hepatocytes (HNF4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).

Organoids MARKER EXPRESSION

Pre-mature liver organoids show liver lobules-like structure.

Organoid Activity

Organoids ORGANOID ACTIVITY

Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.

Organoids ORGANOID ACTIVITY

Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.

 

前沿进展

 
 
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