膜杰作
Star Staining
表达区间及表达系统（Source）
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (GLO-V5243) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Gln 466 & Val 31 - Arg 278 & Arg 24 - Leu 720 (Accession # P12824 & P16832 & P16750).
Predicted N-terminus: His
蛋白结构（Molecular Characterization）
The protein has a calculated MW of 162.2 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) is more than 85% and the molecular weight of this protein is around 195-235 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) immobilized on CM5 Chip can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with an affinity constant of 2.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2). The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα). Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.
