膜杰作
Star Staining
抗体来源（Source）
Monoclonal NeuN/Rbfox3 Antibody, Mouse IgG1 is a mouse monoclonal antibody produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse.
Gene Synonyms: Fox-3, Hrn, Hrnbp3, Rbfox3, NeuN, Neuna60, RNA binding protein, fox-1 homolog (C. elegans) 3.
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1
种属反应性（Reactivity）
Mouse, rat; Others have not been tested
免疫原（Immunogen）
Mouse Rbfox3 peptide fused to GST.
特异性（Specificity）
This product is a specific antibody specifically reacts with NeuN/Rbfox3.
应用（Application）
| Application | Recommended Usage |
| IF | 1:500 |
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, 50 mM Glycine, 50 mM Tris with 40% glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Shipped at -20°C. Store at -20°C for 3 years;
- Shipped at -20°C. Store at -80°C for 5 years.
活性（Bioactivity）-Immunostaining
2D cell staining: Immunofluorescent staining (10X) of Cerebral Organoid-derived neurons (CIPO-BWL001K) labeling NeuN (Red) with purified NE3-S454 at 1:500 dilution. DAPI (blue) was used as nuclear counterstain.
Protocol
背景（Background）
The neuronal nuclear protein (NeuN) is a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. NeuN emerges during early embryogenesis in postmitotic neuroblasts and remains in differentiating and terminally differentiated neurons throughout the whole subsequent ontogeny. NeuN localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals, has been widely used as a marker for post-mitotic neurons.
Gusel'nikova VV, Korzhevskiy DE. Acta Naturae. 2015;7(2):42-7. PMID: 26085943; PMCID: PMC4463411.
General Notes: FOR RESEARCH USE ONLY.
