膜杰作
Star Staining
抗体来源（Source）
Polyclonal Olig2 Antibody, Rabbit IgG is a polyclonal antibody purified from rabbit serum.
Gene Synonyms: Oligo2, bHLHe19, oligodendrocyte transcription factor 2.
种属（Species）
Rabbit polyclonal antibodies
亚型（Isotype）
Rabbit IgG
抗体类型（Antibody Type）
Polyclonal Antibody
种属反应性（Reactivity）
Mouse, rat, human, chicken, dog; Others have not been tested
免疫原（Immunogen）
Mouse Olig2 peptide fused to GST.
特异性（Specificity）
Usually, it is easier to get a positive signal in sections of juvenile animals than that of adults. This antibody does not recognize Olig1 and Olig3.
应用（Application）
| Application | Recommended Usage |
| IF | 1:200 |
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, 50 mM Glycine, 50 mM Tris with 40% glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Shipped at -20°C. Store at -20°C for 3 years;
- Shipped at -20°C. Store at -80°C for 5 years.
活性（Bioactivity）-Immunostaining
2D cell staining: Immunofluorescent staining (10X) of cerebral organoid-derived neurons (CIPO-BWL001K) labeling Olig2 (Red) with purified OL2-S456 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
Protocol
3D organoid staining: Immunofluorescent staining (4x or 10X) of cerebral organoid-(CIPO-BWL001K) labeling Olig2 (Green) with purified OL2-S456 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain. TUJ1 (red) is a neural differentiation marker commonly used to label neural processes.
Protocol
背景（Background）
The oligodendrocyte transcription factor 2 (Olig2) is a basic helix-loop-helix transcription factor which is highly expressed in the oligodendrocyte-lineage cells and in oligodendroglial tumors of the brain as well. Olig2 is an essential regulator of ventral neuroectodermal progenitor cell fate, especially regulating key stages of early oligodendrocyte development. Olig2 has also been reported to act as a gene repressor to determine the cell fate of motor neurons in the spinal cord. Olig2 is a useful marker for primary and mature oligodendrocytes as well as a marker of malignant glioma.
Zhang K, et al. Nat Commun. 2022;13(1):1423. doi: 10.1038/s41467-022-29068-z.
Ono K, et al. Mol Cells. 2009;27(4):397-401. doi: 10.1007/s10059-009-0067-2.
General Notes: FOR RESEARCH USE ONLY.
