膜杰作
Star Staining
分子别名（Synonym）
GDF-8, Myostatin, GDF8, MSTN, Growth,differentiation factor 8
表达区间及表达系统（Source）
Human GDF-8 Protein, Tag Free (GD8-H5344) is expressed from CHO cells. It contains AA Asp 267 - Ser 375 (Accession # O14793).
Predicted N-terminus: Asp 267
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 12.4 kDa. The protein migrates as 12.0 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM HAC, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human GDF-8 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human GDF-8 Protein, Tag Free (Cat. No. GD8-H5344) at 1 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, Fc,Avitag (Cat. No. ACB-H82F3) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized Human GDF-8 Protein, Tag Free (Cat. No. GD8-H5344) at 2 μg/mL (100 μL/well) can bind Human Follistatin Protein, Fc Tag (Cat. No. FON-H5256) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Immobilized Human GDF-8 Protein, Tag Free (Cat. No. GD8-H5344) at 1 μg/mL (100 μL/well) can bind Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
背景（Background）
Growth differentiation factor 8 (GDF8), also known as myostatin, is a unique member of the transforming growth factor-β superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions. GDF8 acts as a negative regulator of skeletal muscle growth and differentiation. In addition to the expression in the musculoskeletal system, GDF8 is also expressed in various tissues, including the reproductive system.
