膜杰作
Star Staining
分子别名（Synonym）
CD4,CD4mut,LEU3
表达区间及表达系统（Source）
Human CD4 Protein, Fc Tag (CD4-H5259) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782).
Predicted N-terminus: Lys 26
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.5 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.2 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) is more than 95% and the molecular weight of this protein is around 150-183 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) at 1 μg/mL (100 μL/well) can bind Ibalizumab (Human IgG4) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade B/C (CN54)] GP120, His Tag (Cat. No. GP4-V15227) at 2 μg/mL (100 μL/well) can bind Human CD4 Protein, Fc Tag (Cat. No. CD4-H5259) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
背景（Background）
T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
