膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RIPO-IWM006-C01 | Intestinal organoid Basal medium MM | 225ml |
| ID | Components | Size |
| RIPO-IWM006-1-C01 | Intestinal organoid Supplement MM | 25ml |
产品描述（Product Details）
Intestinal organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human Intestinal regions. Human iPSC-Derived Intestinal Organoid Maintenance Kit allows long-term maturation and maintenance of the Intestinal organoids.
产品特征（Product Specification）
The basic medium of this kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect organoid differentiation.
存储（Storage）
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.
产品示意（Product Diagram）
Protocol Diagram of Intestinal organoid differentiation.
验证数据（Validation Data）
Organoid Histology and State
The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.
Orgnaoid Histology
Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 Intestinal organoids.
Marker Expression
The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2), brush borders (Villin) and endothelial cells (CD31).
The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).
The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).
The Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).
Organoid Activity
Intestinal organoids differentiated using the Human iPSC-Derived Intestinal Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.
