膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RES036-C01 | Pre-coated Anti-4-1BB Ligand Antibody Microplate | 1 plate |
| RES036-C02 | Human 4-1BB Ligand Standard | 20 μg |
| RES036-C03 | Biotin-Anti-4-1BB Ligand Antibody | 20 μg |
| RES036-C04 | Streptavidin-HRP | 50 μL |
| RES036-C05 | 10×Washing Buffer | 50 mL |
| RES036-C06 | 2×Dilution Buffer | 50 mL |
| RES036-C07 | Substrate Solution | 12 mL |
| RES036-C08 | Stop Solution | 7 mL |
背景（Background）
Human 4-1BB Ligand Kit is based on ELISA sandwich method and designed to measure human 4-1BB Ligand levels in cell culture supernates, serum, and plasma. It contains recombinant human 4-1BB Ligand and a pair of antibodies against the recombinant human 4-1BB Ligand, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of GMP human 4-1BB Ligand (ACROBiosystems, cat#GMP-41LH26) concentrations. The specificity has been verified.
应用说明（Application）
The kit is developed for the detection and quantitative determination of 4-1BB Ligand in human serum and cell culture supernates.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human 4-1BB Ligand. The kit consists of Pre-coated Anti-4-1BB Ligand Antibody Microplate and Human 4-1BB Ligand Standard and Biotin-Anti-4-1BB Ligand Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human 4-1BB Ligand as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-4-1BB Ligand Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
背景（Background）：4-1BB Ligand
Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
