分子别名(Synonym)
IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha
表达区间及表达系统(Source)
Biotinylated Human IL-15 R alpha Protein, His,Avitag (ILA-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1).
Predicted N-terminus: Ile 31
Request for sequence
蛋白结构(Molecular Characterization)
![IL-15 R alpha Structure](/images/structure_Images/ILA-H82E5-STC2.jpg)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22 kDa. The protein migrates as 43-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
![IL-15 R alpha SDS-PAGE](images/sds/ILA-H82E5.jpg)
Biotinylated Human IL-15 R alpha Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
![IL-15 R alpha SEC-MALS](images/mals/ILA-H82E5-M1.jpg)
The purity of Biotinylated Human IL-15 R alpha Protein, His,Avitag (Cat. No. ILA-H82E5) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
![IL-15 R alpha ELISA](images/elisa/ILA-H82E5-E1.jpg)
Immobilized Human IL-15, His Tag (Cat. No. IL5-H52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha Protein, His,Avitag (Cat. No. ILA-H82E5) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景(Background)
IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL-15RA, also known as CD215, IL-15 R alpha, is a widely expressed 60 kDa transmembrane glycoprotein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. An interleukin (IL)-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells, and has shown significant anti-tumor. Signal transduction involves SYK. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.