产品描述(Product Details)
SUMO Protease is a recombinant form of Saccharomyces cerevisiae protease produced in Escherichia coli. SUMO protease can recognize the SUMO tertiary structure and cleave it, has high specificity, and there would be no amino acid residues. This product contains His-tag which can be removed by Ni2+ affinity chromatography resin.
应用说明(Application)
Remove SUMO-tag in any recombinant SUMO fusion protein.
单位定义(Unit Definition)
One unit of SUMO Protease cleaves ≥ 85% of 2 μg control substrate in 1 h at 30℃.
纯度(Purity)
>95% as determined by SDS-PAGE.
酶活(Enzyme Activity)
>10 U/μL
内毒素(Endotoxin)
Less than 0.2 EU per μg by the LAL method.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in 250 mM NaCl, pH 8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储(Storage)
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt.
- -20°C for 3 months under sterile conditions.
电泳(SDS-PAGE)
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)
One unit of SUMO Protease cleaves ≥ 85% of 2 μg control substrate in 1 h at 30℃ (QC tested).