膜杰作
Star Staining
描述（Description）
The Human BMP (Luc) HEK293 Reporter Cell was engineered to express Smad signaling response element driving luciferase expressing systems. When stimulated with BMP protein, receptor-mediated signaling can drive Smad-mediated luminescence.
应用说明（Application）
• Bioactivity detection of human BMP protein
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Signaling Bioassay
Response to human BMP-2 protein (RLU).
The Human BMP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human BMP-2 protein (Cat.No.BM2-H4117). The EC50 was approximately 0.1212 μg/mL.
Protocol
Response to human BMP-2 protein (FOLD).
The Human BMP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human BMP-2 protein (Cat.No.BM2-H4117). The max induction fold was approximately 19.39.
Protocol
Response to human BMP-4 protein (RLU).
The Human BMP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human BMP-4 protein. The EC50 was approximately 0.2787 μg/mL.
Protocol
Response to human BMP-4 protein (FOLD).
The Human BMP (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human BMP-4 protein. The max induction fold was approximately 17.07.
Protocol
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背景（Background）
Human bone morphogenetic protein 2 (BMP2) is also known as human BMP-2, BMP-2, BMP2, BMP 2, h-BMP-2, rh-BMP-2, recombinant human BMP-2, recombinant BMP-2, BMP, is a member of the BMP subgroup belonging to the TGF-b superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. As implied by its name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
