膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RAS198-C01 | Pre-coated HSV-2 (strain 333) Envelope Glycoprotein D (gD) Microplate | 1 plate |
| RAS198-C02 | HSV-2 (strain 333) (gD) Antibody Positive Control | 100 μL |
| RAS198-C03 | HSV-2 (strain 333) (gD) Antibody Negative Control | 100 μL |
| RAS198-C04 | HRP-Conjugated Antibody | 50 μL |
| RAS198-C05 | 10×Washing Buffer | 50 mL |
| RAS198-C06 | Dilution Buffer | 50 mL |
| RAS198-C07 | Substrate Solution | 12 mL |
| RAS198-C08 | Stop Solution | 7 mL |
背景（Background）
Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein D (gD) is a structural component of the herpes simplex virus type 1 (HSV-1) envelope which is essential for virus entry and fusion with host cells. gD plays an important role by binding to the host receptors such as herpes virus entry mediator (HVEM) and nectin-1, a member of the immunoglobulin (Ig)-like cell adhesion molecules.
应用说明（Application）
The kit is developed for titer measurement of Anti-HSV-2 Envelope Glycoprotein D (gD) antibody IgG in mouse serum.
It is for research use only.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-HSV-2 (strain 333) (gD) antibodies in Mouse serum by HSV-2 (strain 333) Envelope Glycoprotein D (gD). The Kit consists of Pre-coated HSV-2 (strain 333) Envelope Glycoprotein D (gD) Microplate,Positive Control,Negative Control and HRP-conjugated antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
