膜杰作
Star Staining
描述（Description）
The Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell was engineered to not only express STAT3 signaling response element, but also express the receptors full length human IL-22 R alpha 1 (Gene ID: 58985) and IL-10 R beta (Gene ID: 3588). When stimulated with human IL-22 protein, receptor-mediated signaling can drive STAT3-mediated luminescence. Neutralization of biological effect of human IL-22 protein by corresponding antibody results in a decrease in luminescence.
应用说明（Application）
• Screen for neutralizing antibodies blocking the stimulation of human IL-22 protein.
• Bioactivity detection of human IL-22 fusion protein.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (40 μg/mL) + Zeocin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Co-expression analysis of human IL-22 R alpha 1 and IL-10 R beta on Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell or negative control cell using anti-human IL-22 antibody followed by staining with PE anti-human IgG Fc antibody and APC-labeled anti-IL-10 R beta antibody.
Protocol
Application
Inhibition of human IL-22 protein-induced reporter activity by anti-human IL-22 neutralizing antibody.
The Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of human IL-22 protein (Cat.No.IL2-H524a) with a final concentration of 0.0007 μg/mL. The EC50 of anti-human IL-22 neutralizing antibody is approximately 0.005372 μg/mL.
Protocol
Signaling Bioassay
Response to human IL-22 protein (RLU).
The Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-22 protein (Cat.No.IL2-H524a). The EC50 was approximately 0.001398 μg/mL.
Protocol
Response to human IL-22 protein (FOLD).
The Human IL-22 R alpha 1/IL-10 R beta (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-22 protein (Cat.No.IL2-H524a). The max induction fold was approximately 66.70.
Protocol
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背景（Background）
The receptor for IL-22, a heterodimeric complex of IL-22RA1 and IL-10RB, binds IL-22, activating signaling pathways that induce genes important for cellular proliferation, inhibition of apoptosis and tissue specific genes, that can include antimicrobial proteins and mucins.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
