分子别名(Synonym)
5-HT2A, HTR2A, HTR2, 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A
表达区间及表达系统(Source)
Human 5-HT2AR Protein (S162K, M164W, S372N), Flag,His Tag (5HR-H55D3) is expressed from Baculovirus-Insect cells. It contains AA Asp 2- Ala 265 & Gln 313 - Lys 403 (Accession # P28223-1 (S162K, M164W, S372N)).
Predicted N-terminus: Asp
蛋白结构(Molecular Characterization)
This protein carries flag tag at the N-terminus and polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.3 kDa.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5, CHS with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性(Bioactivity)-ELISA
![5-HT2AR ELISA](images/elisa/5HR-H55D3-E1.jpg)
Immobilized Human 5-HT2AR Protein (S162K, M164W, S372N), Flag,His Tag (Cat. No. 5HR-H55D3) at 5 μg/mL (100 μL/well) can bind Anti-5-HT2AR Antibody (Cat. No. 5HR-H55D3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
背景(Background)
This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepres.