膜杰作
Star Staining
产品描述（Product Details）
Bst DNA Polymerase is a recombinant form of Bacillus stearothermophilus DNA Polymerase, Large Fragment produced in Escherichia coli. Bst DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity.
应用说明（Application）
Isothermal amplification (LAMP, RT-LAMP)
DNA sequencing of rich GC sequence
Rapid sequencing of DNA templates (ng)
单位定义（Unit Definition）
One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
纯度（Purity）
>90% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>8 U/μL
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 10 mM Tris-HCl, pH7.1 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt.
- -20°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）
One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C (QC tested).
