膜杰作
Star Staining
分子别名（Synonym）
EPOR
表达区间及表达系统（Source）
Human Erythropoietin R Protein, His Tag (EPR-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Pro 250 (Accession # P19235-1).
Predicted N-terminus: Ala 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 30-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Erythropoietin R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) is more than 85% and the molecular weight of this protein is around 25-38 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) immobilized on CM5 Chip can bind Human EPO Protein, premium grade (Cat. No. EPO-H4214) with an affinity constant of 0.142 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) inhibits human erythropoietin/EPO-dependent proliferation of TF-1 cells. The EC50 is 211.1 ng/mL (Routinely tested).
Protocol
背景（Background）
This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphat.
