膜杰作
Star Staining
分子别名（Synonym）
FGF-6,Fibroblast growth factor 6,HST-2,HBGF-6
表达区间及表达系统（Source）
Human FGF-6 Protein, His Tag (FG6-H51H4) is expressed from E. coli cells. It contains AA Gly 41 - Ile 208 (Accession # P10767-1).
Predicted N-terminus: Gly 41
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.6 kDa. The protein migrates as 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Sodium Citrate, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human FGF-6 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human FGF-6 Protein, His Tag (Cat. No. FG6-H51H4) at 5 μg/mL (100 μL/well) can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with a linear range of 0.039-1.25 μg/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human FGF-6 Protein, His Tag (Cat. No. FG6-H51H4) immobilized on CM5 Chip can bind Human FGF R1, His Tag (Cat. No. FG1-H5223) with an affinity constant of 45 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF-6 Protein, His Tag (Cat. No. FG6-H51H4) immobilized on CM5 Chip can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with an affinity constant of 13.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF-6 Protein, His Tag (Cat. No. FG6-H51H4) immobilized on CM5 Chip can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 33.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Fibroblast growth factor 6 plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal muscle regeneration.
For instance, FGF-6 might involve in MSCs proliferation and chondrogenic differentiation. Additionally, FGF-6 were identified as oncogene products. FGF6 is found over expressed in prostatic intraepithelial neoplasia and prostate cancer and promotes the proliferation of the transformed prostatic epithelial cells.
