膜杰作
Star Staining
优势与特点（FAB）
- Rapid - Results can be obtained within 90 minutes.
- Reference Standard Traceability - The reference standard is traceable to the National Standard NIFDC (cat # 140619).
- Rigorous Method Validation - Validation reports compliant with ICH guidelines can be provided upon request.
- High Sensitivity - Sensitivity for Bovine Serum Albumin (BSA) is < 0.5 ng/mL.
- Strong Specificity - No cross-reactivity with non-target proteins.
- Excellent Buffer Compatibility - Compatible with a wide range of buffer systems.
产品参数（Product Specifications）
| Assay Type | Sandwich-ELISA |
| Analyte | BSA (Bovine serum albumin) |
| Format | 96T(8×12 strips) |
| Regulatory Status | RUO |
| Sensitivity | ＜0.5ng/mL |
| Standard Curve Range | 0.5ng/mL-40.5ng/mL |
| Assay Time | 1.5 hr |
| Suitable Sample Type | For the quantitative determination of BSA (Bovine serum albumin) |
| Sample volume | 50μL |
产品概述（Product Overview）
resDetect™ BSA ELISA Kit is based on the ELISA sandwich method and is designed for the detection and quantitative determination of Bovine Serum Albumin (BSA) in biological products. This kit is used as a tool to support optimal purification process development and routine quality control of in-process streams and final products in various biotechnological processes, such as cell and tissue culture. The standard BSA has been calibrated against the NIFDC standard (cat # 140619) to ensure accurate assay results. It can also be used as a universal detection tool to quantitative determination of BSA.
应用说明（Application）
0
It is for research use only.
存储（Storage）
组分（Materials Provided）
| ID | Components | Size |
| RES01-C01 | Pre-Coated Anti-BSA Antibody Microplate | 1 plate(8×12 strips) |
| RES01-C02A | BSA (Bovine serum albumin) Standard (200ng/mL) | 500 μL |
| RES01-C02B | BSA (Bovine serum albumin) Standard 5 (40.5ng/mL) | 500 μL |
| RES01-C02C | BSA (Bovine serum albumin) Standard 4 (13.5ng/mL) | 500 μL |
| RES01-C02D | BSA (Bovine serum albumin) Standard 3 (4.5ng/mL) | 500 μL |
| RES01-C02E | BSA (Bovine serum albumin) Standard 2 (1.5ng/mL) | 500 μL |
| RES01-C02F | BSA (Bovine serum albumin) Standard 1 (0.5ng/mL) | 500 μL |
| RES01-C02G | BSA (Bovine serum albumin) Standard 0 (0ng/mL) | 500 μL |
| RES01-C03 | HRP-Anti-BSA Antibody | 100 μL |
| RES01-C04 | 1×Sample Dilution Buffer | 50 mLX2 |
| RES01-C05 | 20×Washing Buffer | 25 mL |
| RES01-C06 | Substrate Solution | 12 mL |
| RES01-C07 | Stop Solution | 8 mL |
典型数据-Typical Data Please refer to DS document for the assay protocol.
Detection of BSA ( Bovine serum albumin) by ELISA Assay.
Immobilized Anti-BSA Antibody can bind BSA (Bovine serum albumin) Detection was performed using HRP-Anti- BSA Antibody with sensitivity of 0.5 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
验证（Validation）
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
准确度（Accuracy）
Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.
干扰效应(Interference effect)
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
专属性（Specificity）
High, medium and low concentrations of BSA were added to Human serum (v/v 1%), Horse serum (v/v 10%) , Human Serum Albumin (1.25 mg/mL) and Rabbit serum (v/v 10%), the recovery rate of BSA was used as the specific validation index.
High, medium and low concentrations of BSA were added to MDCK( 2×106 cells/mL), HEK293( 3.5×106 cells/mL) , CHO( 2×106 cells/mL) and T-lymphocyte( 2.15×106 cells/mL) the recovery rate of BSA was used as the specific validation index.
