膜杰作
Star Staining
分子别名（Synonym）
CC chemokine receptor 8,CD198,ChemR1,CKRL1,CKR-L1,CKRL1,CY6,GPRCL1,GPRCY6,GPR-CY6,TER-1
表达区间及表达系统（Source）
Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (CC8-H52D6) is expressed from human 293 cells (HEK293). It is a membrane protein, contains complete wild type 7-transmembrane domans (TMD) and extracellular doman (ECD).
Predicted N-terminus: Asp
蛋白结构（Molecular Characterization）
The protein has a calculated MW of 62.0 kDa.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of LMNG.
LMNG and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove LMNG and CHS.
LMNG/CHS buffer (LG-13) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, LMNG, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) at 0.5 μg/mL (100 μL/well) can bind Anti-CCR8 Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) at 5 μg/mL (100 μL/well) can bind Human CCL1, Fc Tag (Cat. No. CC1-H5254) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-CCR8 captured on Protein A Chip can bind Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) with an affinity constant of 63.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
This entry represents CC chemokine receptor 8 (CCR8), which it is expressed predominantly in lymphoid tissues and has also been found in glomerular podocytes and human umbilical vein endothelial cells (HUVECs). CCR8 is associated with Th2 lymphocytes, which are critical for allergy, and has a role in lymphocyte activation, migration, proliferation and differentiation and in allergic diseases. CCR8 binds to CCL1 (also known as I-309) and to CCL16 (also known as liver expressed chemokine). It also exhibits a high affinity for three chemokines of viral origin: vMIP-I, vMIP-II and vMCC-I.
